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Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

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  • Author(s)Awtar Krishan / H. Krishnamurthy / Satish Totey
  • Edition1
  • Published28th July 2010
  • PublisherJohn Wiley & Sons Inc (US)
  • ISBN
  • University course code
A much-needed primer on the use of laser flow cytometry for stem
cell analysis

Laser flow cytometry is a powerful tool for rapid analysis of
cells for marker expression, cell cycle position, proliferation,
and apoptosis. However, no resources specifically address the use
of this methodology for the study of stem cells; this is especially
important as stem cell analysis involves specialized methods and
staining procedures based on specific characteristics such as
marker expression, cell size, drug transport, and efflux of the
stem cells.

Now, this book reviews these procedures, discusses the science
behind them, and provides real-world examples to illustrate the
usefulness of the methods. It brings together world-class experts
in pathology, biophysics, immunology, and stem cell research, who
draw upon their extensive experience with the methods and show
examples of good data to help guide researchers in the right

Chapter coverage includes:

  • Stem cell analysis and sorting using side population

  • Flow cytometry in the study of proliferation and apoptosis

  • Stem cell biology and application

  • Identification and isolation of very small embryonic-like stem
    cells from murine and human specimens

  • Hematopoietic stem cells?issues in enumeration

  • Human embryonic stem cells: long-term culture and
    cardiovascular differentiation

  • Limbal stem cells and corneal regeneration

  • Flow cytometric sorting of spermatogonial stem cells

  • Breast cancer stem cells

  • Stem cell marker expression in cells from body cavity

This book is an essential resource for all graduate students,
practitioners in developing countries, libraries and book
repositories of universities and research institutions, and
individual researchers. It is also of interest to laboratories
engaged in stem cell research and use of stem cells for tissue
regeneration, and to any organization dealing in stem cell and
tissue regeneration research.

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